Immunological experiments and techniques

Subject description

Planning of immunological experiments. Overview of immunological methods and techniques and the selection of those that candidate encountered in the implementation of the doctoral dissertation. The candidate can use its own material. Options: Production of monoclonal-polyclonal-recombinant antibodies. Phage display. Conjugation of antibodies and their use for detection / isolation / application. Isolation and detection of antigens: DIBA, Westrn-blott, ELISA, immunoprecipitation. Monitoring of immune response at the level of molecules (antibodies, cytokines) and gene expression. Complex experiments using cDNA microarray and phenotypic microarrays. Cell models to study the effects of antigens.

The subject is taught in programs

Objectives and competences

Candidate will design the protocol for experiment and acquire skills of planning, implementation, evaluation and presentation of results.

Teaching and learning methods

Practical work in the laboratory, supervised by the lecturer and consultations.

Expected study results

Knowledge and understanding:

Understanding the complexity of immunological studies. Ability to design immunological experiments. Practical experience of work in the immunological laboratory. The result, which is part of the doctoral thesis.

Basic sources and literature

Tekoča znanstvena periodika

Scientific papers

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University of Ljubljana, Faculty of Electrical Engineering Tržaška cesta 25, 1000 Ljubljana

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