Subject description
Estrus synchronization and superovulation Overview of methods for sexual cycle synchro- nization in different animal species and procedures for superovulation−Harvesting of oocytes−Oocyte retrieval by in vitro follicle puncture, morphological assessment of oocytes and assessment of developmental potential−In vitro maturation and in vitro fertilization In vitro egg maturation, seed capacity and in vitro fertilization.−Cultivation of preimplantation embryos Cultivation of embryos from fertilization to budding blastocyst, monitoring of morphological changes, cell cycle regulation−Isolation of individual cells from 2-8 cell embryos−Microsurgical isolation of individual cells from preimplantation embryos, cultivation and isolation of total RNA−Single cell genotyping (scPCR) Genetic diagnostics of individual cells−(gender, hereditary defects), mosaicism, maternal inheritance−Microsurgical procedures−Mechanical and ultrasonic embryo division, embryonic cell transfer, microinjection−Transcriptomics of individual cells, Quantification of maternal and embryonic gene expression, monitoring of transcriptomic dynamics
The subject is taught in programs
Objectives and competences
Students learn the basic procedures associated with biotechnological measures in the reproduction of domestic animals. They become acquainted with methods for obtaining, culturing and manipulating embryos. They combine this knowledge with bioinformatics approaches and analyzes to study gene expression in the preimplantation state using genomic diagnostic methods. The comparative approach enables the development of adapted methods for genetic / genomic monitoring of the regulation of gene expression from fertilization to implantation.
Teaching and learning methods
Seminars, consultations, laboratory exercices.
Expected study results
Knowledge and understanding:
Students gain basic knowledge about early embryogenesis and biotechnological methods for its manipulation. In addition, they get technical and bioinformatic knowledge for the survey of gene expression in preimplantational embryos.
Basic sources and literature
Heiner Niemann, Christine Wrenzycki (2018): Animal Biotechnology 1, Reproductive Biotechnologies ISBN 978-3-319-92327-7, DOI 10.1007/978-3-319-92327-7, Springer International Publishing AG. Juan Carlos Gardón and Katy Satué (2021) Biotechnologies Applied to Animal Reproduction. ISBN 9781771888714, Apple Academic Press. Jose L. Estrada (2019) Biotechnology of Reproduction Special issue of Animals ISSN 2076-2615
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University of Ljubljana, Faculty of Electrical Engineering Tržaška cesta 25, 1000 Ljubljana