Subject description
– Correlative microscopy with detailed insight into the structure and function of the same cells.
– Simultaneous observation of a given subcellular structure and cellular processes from the micron to the nanometer scale while maintaining spatial orientation.
– Labelling markers in correlative microscopy.
– Introduction to the different correlative microscopy approaches and their applications.
– The correct selection of the particular correlative microscopy approaches: their advantages and disadvantages.
Demonstration of the correlative microscopy from the methods of culturing the cells, methods in light and electron microscopy to the semi/fully automated sample analyses.
The subject is taught in programs
Objectives and competences
- Acquiring of special knowledge on correlative microscopy.
- To learn and understand the correlative microscopy for observation and analysis of the same areas within the cell.
Teaching and learning methods
Lectures, consultations, practical demonstrations, seminars and individual project.
Expected study results
Knowledge and understanding:
- Practical knowledge to analyse cell ultrastructure and function using correlative microscopy.
- Choosing of appropriate methodology of correlative microscopy for applications in cell biology studies.
Basic sources and literature
- Correlative Light and Electron Microscopy IV, 2021, Volume 162 (Methods in Cell Biology), Thomas Mueller-Reichert (Editor), Paul Verkade (Editor), pages 2-430.
Najnovejši pregledni in raziskovalni članki s področja korelativne mikroskopije /The latest review and research articles from the field of correlative microscopy.
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University of Ljubljana, Faculty of Electrical Engineering Tržaška cesta 25, 1000 Ljubljana