Subject description
Modern engineering trends and isolation of biological macromolecules (such as enzymes, monoclonal antibodies, plasmid DNA), viruses (bacteriophages and human viruses) and cells (stem cells) will be presented, which are important in the field of safety, nutrition and health.
The main focus of the course will be on various isolation techniques of the considered bioproducts based on precipitation, extraction with two-phase aqueous systems, chromatography, including modern trends in continuous processes and their use in bioconversion, with an emphasis on microreactors.
Students will be familiar with the basics of experimental design with an emphasis on QbD (Quality by Design), regulatory aspects and related specific analytics e.g. PAT (Process Analytical Technology). Specific examples of processes will also be presented.
Other lecturers may be involved for specific areas.
The subject is taught in programs
Objectives and competences
- Understanding theoretical principles of techniques used for production of biologic molecules, viruses and cells
- Understanding concepts of experimental design for downstream processing
- Microreactors and continuous processes
- Understanding design and performance of biocatalytic processes
- Understanding of regulatory concepts
Teaching and learning methods
- lectures
- seminars (regular presentations with discussions)
Expected study results
- Implementation of acquired knowledge in projects
- Demonstrated achievement of educational goals
Basic sources and literature
Izbrana poglavja iz Carta G., Jungbauer A. Protein Chromatography: Process Development and Scale-Up, Wiley-VCH, Weinheim, 2010
Izbrana poglavja iz Subramanian G. (Eds.). Biopharmaceutical production technology. Weinheim: Wiley-VCH, 2012
Izbrana poglavja iz Hessel, V., Renken, A., Schouten, J.C., Yoshida, J.-I. (2009) editors, Micro Process Engineering: A Comprehensive Handbook, Vol. 1-3, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany
Izbrana poglavja iz Buchholz K., Kasche V., Bornscheuer U.T. (2012) Biocatalysts and Enzyme Technology, 2nd Edition
Znanstveni članki iz tekoče znanstvene perodike.
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University of Ljubljana, Faculty of Electrical Engineering Tržaška cesta 25, 1000 Ljubljana